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nematodes from the soil and soybean roots and counting them ? is
time-consuming and labor-intensive. In the lab, Zhang infects soybean plants by
putting nematode eggs into the soil they’re growing in. He waits 30-35 days for the
nematode to infect the plant and develop to adult stages, tracks its path from the
roots and manually counts the number of parasites to quantify the level of infection.
“Basically, with automated phenotyping, we’re trying to make this a faster process,
targeting the nematode infection and detecting it earlier, maybe in 10 to 14 days
after nematode inoculation. The other thing is, the phenotyping is non-destructive,”
he says. “We don’t have to take down the plant and count the nematodes.”
Nematode infection of roots can negatively impact the photosynthesis efficacy.
Chlorophyll fluorescence imaging can be used to measure photosynthesis efficiency
of soybean leaves. Hyperspectral imaging looks at wavelength patterns and possibly
emissions associated with a nematode infection. “We’re using both to see which
one works better,” Zhang says. Instead of roots, the hyperspectral and fluorescence
imaging methods focus on leaves. Even before root symptoms become obvious, the
parasitic infection reduces photosynthesis. “This might be a subtle change ? our
eyes may not catch it ? but using this kind of the hyperspectral imaging or
fluorescence camera, we might be able to catch it,” Zhang says. “Based on leaf
response, we try to tell if there is a nematode infection on soybean roots in the
soil and the level of infection.” Hyperspectral imaging in the phenomics center
focuses on different wavelengths that somehow may be associated with a nematode
infection. For example, the researchers are studying if leaf reflections at certain
wavelength ranges of infected plants is higher or lower than control plants with no
infection. Zhang’s team might be able to reduce the time required for SCN detection
to within 10 days of SCN inoculation in a controlled environment, so the project is
still ongoing, he says. Still, the study shows promise for soybean resistance
discovery and breeding purposes. Next steps include testing different levels of the
nematode inoculums, different soybean varieties or breeding lines.
- 419 -
time-consuming and labor-intensive. In the lab, Zhang infects soybean plants by
putting nematode eggs into the soil they’re growing in. He waits 30-35 days for the
nematode to infect the plant and develop to adult stages, tracks its path from the
roots and manually counts the number of parasites to quantify the level of infection.
“Basically, with automated phenotyping, we’re trying to make this a faster process,
targeting the nematode infection and detecting it earlier, maybe in 10 to 14 days
after nematode inoculation. The other thing is, the phenotyping is non-destructive,”
he says. “We don’t have to take down the plant and count the nematodes.”
Nematode infection of roots can negatively impact the photosynthesis efficacy.
Chlorophyll fluorescence imaging can be used to measure photosynthesis efficiency
of soybean leaves. Hyperspectral imaging looks at wavelength patterns and possibly
emissions associated with a nematode infection. “We’re using both to see which
one works better,” Zhang says. Instead of roots, the hyperspectral and fluorescence
imaging methods focus on leaves. Even before root symptoms become obvious, the
parasitic infection reduces photosynthesis. “This might be a subtle change ? our
eyes may not catch it ? but using this kind of the hyperspectral imaging or
fluorescence camera, we might be able to catch it,” Zhang says. “Based on leaf
response, we try to tell if there is a nematode infection on soybean roots in the
soil and the level of infection.” Hyperspectral imaging in the phenomics center
focuses on different wavelengths that somehow may be associated with a nematode
infection. For example, the researchers are studying if leaf reflections at certain
wavelength ranges of infected plants is higher or lower than control plants with no
infection. Zhang’s team might be able to reduce the time required for SCN detection
to within 10 days of SCN inoculation in a controlled environment, so the project is
still ongoing, he says. Still, the study shows promise for soybean resistance
discovery and breeding purposes. Next steps include testing different levels of the
nematode inoculums, different soybean varieties or breeding lines.
- 419 -
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